Impact of chronic lead exposure on metal distribution and biological effects to periphyton

Chronic Pb exposure microcosm studies were carried out on two different periphyton communities over the course of 3 weeks to link Pb distribution to biological effects in periphyton. We show that three-week exposures of periphyton to 20.6 ± 0.4 μM PbT (330 nM Pb(2+)) did not have observable biological effects on photosynthesis, respiration, extracellular enzymatic activities, or biomass accrual. Metal distribution studies showed that the majority of Pb was associated with the operationally defined sorbed and non-EDTA-exchangeable fractions, and relatively little with extracellular polymeric substances (EPS). No significant effects of Pb on Fe and Mn distributions were observed, whereas higher Cu accumulation occurred from increased free Cu(2+) in the exposure medium. High Fe:C and Mn:C ratios indicated the presence of inorganic Fe and Mn material associated with the non-EDTA-exchangeable fraction, which likely sequesters Pb and explains the absence of measurable biological effects. Although no toxic effects of Pb were observed on the periphytic organisms themselves, periphyton can be a significant source of Pb to grazing organisms in aquatic ecosystems.