Total internal reflection fluorescence microscopy of intraflagellar transport in Tetrahymena thermophila
Yu-Yang Jiang1, Karl Lechtreck1, Jacek Gaertig1
1Department of Cellular Biology, University of Georgia, Athens, GA, USA.
Abstract:
Live imaging has become a powerful tool in studies of ciliary proteins. Tetrahymena thermophila is an established ciliated model with well-developed genetic and biochemical approaches, but its large size, complex shape, and the large number of short and overlapping cilia, have made live imaging of ciliary proteins challenging. Here we describe a method that combines paralysis of cilia by nickel ions and total internal reflection microscopy for live imaging of fluorescent proteins inside cilia of Tetrahymena. Using this method, we quantitatively documented the intraflagellar transport in Tetrahymena.
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