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Activity of Andrographolide and Its Derivatives on HPV16 Pseudovirus Infection and Viral Oncogene Expression in

Tipaya Ekalaksananan1, Waree Sookmai, Supada Fangkham

  • 1a Department of Microbiology, Faculty of Medicine, HPV & EBV and Carcinogenesis Research Group, Khon Kaen University , Khon Kaen , Thailand.

Nutrition and Cancer
|April 4, 2015
PubMed

Insights

Andrographolide and its derivatives inhibit human papillomavirus (HPV) infection and cervical cancer progression by suppressing viral oncogene expression and promoting apoptosis.

Area of Science:

  • Phytochemistry
  • Virology
  • Oncology

Background:

  • Andrographolide (Androg) exhibits antiviral and antitumor properties.
  • The impact of Androg and its derivatives on human papillomavirus (HPV) infection and cervical cancer remains unclear.

Purpose of the Study:

  • To investigate the effects of Androg, 14-deoxy-11,12-didehydroandrographolide (14-DDA), and 3,19-isopropylidene andrographolide (IPAD) on HPV16 pseudovirus (HPV16PsV) infectivity.
  • To assess the impact of these compounds on HPV16 E6 oncogene expression and cervical cancer cell apoptosis.

Main Methods:

  • Treatment of C33A, SiHa, and CaSki cells with Androg, 14-DDA, and IPAD.
  • Assay of HPV16PsV infectivity.
  • Measurement of HPV16 long control region (LCR) transcription activity.
  • Quantification of E6 oncogene expression and p53 protein levels.
  • Analysis of cell apoptosis and cell cycle arrest.

Main Results:

  • All tested compounds inhibited HPV16PsV infection, with 14-DDA showing the highest potency.
  • Androg suppressed HPV16 LCR transcription and E6 oncogene expression in a dose-dependent manner.
  • IPAD and Androg treatments restored p53 protein levels, induced cell apoptosis, and caused G2/M phase cell cycle arrest.

Conclusions:

  • Androg and its derivatives demonstrate distinct activities against HPV infection and cervical cancer.
  • These compounds show potential as therapeutic agents for HPV-related diseases and cervical cancer treatment.

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