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Related Experiment Video

Updated: Apr 15, 2026

Neutron Radiography and Computed Tomography of Biological Systems at the Oak Ridge National Laboratory's High Flux Isotope Reactor
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A novel approach to determine post mortem interval using neutron radiography.

Hassina Z Bilheux1, Maria Cekanova2, Arpad A Vass3

  • 1Chemical and Engineering Materials Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, United States.

Forensic Science International
|April 4, 2015
PubMed
Summary

Neutron radiography can estimate post-mortem interval (PMI) by measuring hydrogen loss in decaying tissues. This technique offers a novel, objective method for forensic science to determine time since death.

Keywords:
Canine tissuesHydrogen contentNeutron radiographyPost-mortem interval

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Area of Science:

  • Forensic Science
  • Biophysics

Background:

  • Determining post-mortem interval (PMI) is crucial in forensic investigations.
  • Current PMI methods are sensitive to environmental factors and decomposition variability.
  • Tissue changes after death involve alterations in water content and elemental composition.

Purpose of the Study:

  • To evaluate neutron radiography (NR) as a method for objectively determining PMI.
  • To measure changes in hydrogen (H) content during tissue decomposition.
  • To correlate hydrogen depletion with the time since death.

Main Methods:

  • Canine cadavers were used as a model for human decomposition.
  • Tissues were analyzed using neutron radiography under controlled and uncontrolled environmental conditions.
  • Neutron radiographs were supplemented with photographic and histological data.

Main Results:

  • Increased neutron transmission correlated with decreased hydrogen content in decaying tissues.
  • Tissues with lower hydrogen content appeared brighter in radiographs.
  • Lung and muscle tissues showed greater changes in neutron transmission than bone over 10 days.

Conclusions:

  • Neutron radiography can detect hydrogen abundance changes related to PMI.
  • This technique shows potential for objective PMI determination in forensic science.
  • Further research is needed for uncontrolled environmental conditions.