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Published on: November 10, 2016
Immunosensor for trace penicillin G detection in milk based on supported bilayer lipid membrane modified with gold
Han Li1, Bing Xu1, Danqi Wang1
1Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
Abstract:
In this work, we developed an immunosensor for electrochemical detection of penicillin G at trace level. The biosensor was fabricated by immobilizing anti-penicillin G in a supported bilayer lipid membrane (s-BLM) modified with gold nanoparticles, and the modified electrodes were characterized by the scanning electron microscope (SEM), cyclic voltammetry and electrochemical impedance spectroscopy. The biosensor was able to detect penicillin G with a linear correlation ranging from 3.34×10(-3)ng/L to 3.34×10(3)ng/L and a detection limit of 2.7×10(-4)ng/L, much lower than the maximum residue limit (MRL) of penicillin G in milk (4ppb, equal to 4×10(3)ng/L) set out by the European Union. The mean coefficient variation (CV) of the intra-assays and the inter-assays were 5.4% and 7.7%, respectively. In addition, the concentration of penicillin G in milk samples determined by this biosensor was in good agreement with that determined by high performance liquid chromatography (HPLC) assay.
