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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
A novel ECL biosensor for β-lactamase detection: Using RU(II) linked-ampicillin complex as the recognition element
Guo-Feng Gui1, Ying Zhuo2, Ya-Qin Chai2
1Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China; College of Chemical Engineering, Guizhou University of Engineering Science, Guizhou 551700, China.
A novel electrogenerated chemiluminescence (ECL) biosensor was developed for detecting beta-lactamase. This biosensor utilizes a unique ruthenium-ampicillin complex for sensitive and specific enzyme identification.
Area of Science:
- Electrochemistry
- Biochemistry
- Materials Science
Background:
- Beta-lactamase enzymes are crucial targets in antimicrobial resistance research.
- Developing sensitive and specific biosensors for beta-lactamase detection is essential for clinical diagnostics and drug development.
- Existing detection methods often lack sensitivity or require complex procedures.
Purpose of the Study:
- To develop a simple and highly sensitive electrogenerated chemiluminescence (ECL) biosensor for the determination of beta-lactamase.
- To introduce a novel ruthenium-ampicillin (Ru-Amp) complex as both a specific recognition element and an ECL luminescent reagent.
- To explore the potential of Ru-Amp complex for designing new ruthenium-based ECL luminophores in biosensing.
Main Methods:
- Immobilization of Ru-Amp complex onto gold nanoparticles (TA@AuNPs) via electrostatic adsorption and pi-pi interactions.
- Self-assembly of Ru-Amp/TA@AuNPs nanocomposites onto a carbon nanotubes-nanofiber (CNTs-Nf) modified glassy carbon electrode.
- Utilizing the autonomous hydrolysis of ampicillin by beta-lactamase to trigger ECL emission for detection.
Main Results:
- The developed biosensor demonstrated excellent sensitivity for beta-lactamase detection over a concentration range of 50 pg mL(-1) to 100 ng mL(-1).
- A low limit of detection (LOD) of 37 pg mL(-1) was achieved, indicating high sensitivity.
- The Ru-Amp/TA@AuNPs nanocomposites formed stable films with excellent ECL behaviors.
Conclusions:
- The proposed Ru-Amp complex serves as an effective recognition element and ECL reagent for beta-lactamase detection.
- The ECL biosensor offers a simple, sensitive, and efficient platform for beta-lactamase determination.
- This approach provides a promising avenue for developing novel Ru-based ECL luminophores for advanced biosensing applications.
