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Delftia deserti sp. nov., isolated from a desert soil sample
Chang-Tian Li1, Zheng-Fei Yan, Xiao Chu
1Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, People's Republic of China, lct@jlau.edu.cn.
Antonie Van Leeuwenhoek
|April 6, 2015
Summary
A novel bacterium, Delftia desertisoli sp. nov., was identified from a Chinese desert soil sample. This motile, Gram-negative bacterium expands the known diversity within the Delftia genus.
Area of Science:
- Microbiology
- Bacteriology
- Environmental Science
Background:
- The genus Delftia comprises bacteria known for their metabolic versatility and presence in various environments.
- Soil ecosystems, particularly arid regions like deserts, harbor unique microbial communities with potential novel species.
Purpose of the Study:
- To isolate, characterize, and phylogenetically classify a novel bacterial strain from a desert soil sample.
- To determine the taxonomic position of the novel strain within the genus Delftia and propose a new species.
Main Methods:
- Isolation and cultivation of bacteria from a desert soil sample.
- Gram staining, motility tests, and pigment observation.
- Phylogenetic analysis based on 16S rRNA gene sequencing.
- Chemotaxonomic analyses including major fatty acids and respiratory quinones.
- DNA-G+C content determination and DNA-DNA hybridization experiments.
Main Results:
- A novel Gram-negative, short rod, motile, light brownish-pigmented bacterium, strain YIM Y792(T), was isolated.
- Phylogenetic analysis placed strain YIM Y792(T) within the genus Delftia, with highest 16S rRNA gene similarity to Delftia lacustris.
- Chemotaxonomic data (Q-8, major fatty acids) and polar lipid profiles were consistent with the genus Delftia.
- DNA-DNA relatedness values were below the threshold for species delineation with closely related Delftia species.
- The strain exhibited optimal growth at 30°C, pH 7.0, and 1.0% NaCl, with a G+C content of 70.3 mol%.
Conclusions:
- Strain YIM Y792(T) represents a novel species within the genus Delftia.
- The proposed name for the novel species is Delftia desertisoli sp. nov., with YIM Y792(T) as the type strain.
- This finding contributes to the understanding of microbial diversity in extreme desert environments.
