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Human vascular permeability factor. Isolation from U937 cells.

D T Connolly1, J V Olander, D Heuvelman

  • 1Department of Cell Culture, Monsanto Company, St. Louis, Missouri 63167.

The Journal of Biological Chemistry
|November 25, 1989
PubMed
Summary
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Human vascular permeability factor (hVPF) promotes blood vessel leakage and endothelial cell growth. This protein

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Human vascular permeability factor (hVPF) is a glycoprotein involved in fluid and protein leakage from blood vessels.
  • Its precise function remains unknown, but its bioactivities suggest roles in inflammation, wound healing, and tumor angiogenesis.

Purpose of the Study:

  • To purify and characterize human vascular permeability factor (hVPF).
  • To investigate the biological activities of hVPF, including its effects on vascular permeability and endothelial cell growth.

Main Methods:

  • Purification of hVPF from serum-free conditioned medium of the U937 cell line.
  • Assessment of dermal blood vessel leakage in guinea pigs.
  • In vitro endothelial cell growth assays.
  • Analysis of hVPF forms using pH gradient electrophoresis.

Related Experiment Videos

  • Cross-reactivity studies with antibodies against guinea pig vascular permeability factor.
  • N-terminal amino acid sequencing.

    • Main Results:

    • hVPF was purified as a disulfide-linked dimeric 40-kDa protein.
    • hVPF induced dermal blood vessel leakage in guinea pigs at a dose of 20 ng.
    • hVPF promoted in vitro endothelial cell growth at concentrations as low as 50 pM.
    • Multiple forms of hVPF with pI > 7.5 were identified.
    • Antibodies against guinea pig vascular permeability factor cross-reacted with hVPF.
    • N-terminal amino acid sequence showed similarity to guinea pig vascular permeability factor.

    Conclusions:

    • hVPF exhibits potent bioactivities, including promoting vascular permeability and endothelial cell growth.
    • hVPF shares structural and functional similarities with guinea pig vascular permeability factor.
    • These findings suggest hVPF plays a significant role in physiological and pathological processes involving blood vessels.