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Lactose hydrolysis by β-galactosidase enzyme: optimization using response surface methodology.

Bipasha Das1, Ananda Prasad Roy1, Sangita Bhattacharjee2

  • 1Chemical Engineering Department, Jadavpur University, Kolkata 700032, West Bengal, India.

Ecotoxicology and Environmental Safety
|April 6, 2015
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Summary

This study optimized lactose hydrolysis using β-galactosidase, yielding glucose and galactose. Optimal conditions were determined for both free and immobilized enzyme processes to maximize hydrolysis efficiency.

Keywords:
Calcium alginateImmobilized enzymeLactose hydrolysisResponse surface methodologyβ-galactosidase

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Area of Science:

  • Biotechnology
  • Enzyme Technology
  • Biochemistry

Background:

  • Lactose hydrolysis is crucial for producing glucose and galactose from lactose.
  • β-galactosidase is a key enzyme for this bioconversion.
  • Optimizing hydrolysis conditions enhances product yield and process efficiency.

Purpose of the Study:

  • To optimize lactose hydrolysis using free and immobilized β-galactosidase.
  • To determine the ideal operational parameters for maximizing glucose and galactose production.
  • To compare the efficiency of free versus immobilized enzyme systems.

Main Methods:

  • Response Surface Methodology (RSM) with Central Composite Design (CCD) was utilized.
  • Key parameters optimized included temperature, pH, and enzyme concentration for free β-galactosidase.
  • Sodium alginate and calcium chloride concentrations were optimized for immobilized β-galactosidase.

Main Results:

  • Optimal conditions for free β-galactosidase: 35.5°C, pH 6.7, and 6.7 mg/mL enzyme concentration.
  • Optimal conditions for immobilized β-galactosidase: 3% sodium alginate, 5.9% calcium chloride, and 5.2 mg/mL enzyme concentration.
  • Variance analysis confirmed these parameters maximize lactose hydrolysis.

Conclusions:

  • The study successfully identified optimal conditions for lactose hydrolysis using both free and immobilized β-galactosidase.
  • Immobilization offers an alternative strategy for efficient lactose bioconversion.
  • Further research can explore scaling these optimized conditions for industrial applications.