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[RPN4 the yeast transcription factor promotes the complex defence against methyi, methanesulfonate]

D S Spasskaia, D S Karpov, A S Mironov

    Molekuliarnaia Biologiia
    |April 7, 2015
    PubMed

    Insights

    The transcription factor Rpn4 protects yeast cells from methyl methanesulfonate (MMS) genotoxicity. Rpn4 regulates DNA repair, antioxidant response, and glucose metabolism genes during MMS stress.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Cellular Stress Response

    Background:

    • Methyl methanesulfonate (MMS) is a genotoxic alkylating agent inducing DNA damage and oxidative stress.
    • The transcription factor Rpn4 is known to regulate the ubiquitin-proteasome system (UPS).
    • Previous studies suggest Rpn4 involvement in stress response pathways beyond the UPS.

    Purpose of the Study:

    • To investigate the role of Rpn4 in yeast cells exposed to methyl methanesulfonate (MMS) stress.
    • To elucidate the mechanisms by which Rpn4 mediates protection against MMS toxicity.
    • To identify specific gene targets regulated by Rpn4 during genotoxic stress.

    Main Methods:

    • Exposure of yeast cells to methyl methanesulfonate (MMS).
    • Analysis of gene expression changes under MMS stress.
    • Investigating the regulatory function of the transcription factor Rpn4.

    Main Results:

    • Rpn4 demonstrates a protective role against the toxic effects of MMS in yeast.
    • Under MMS stress, Rpn4 actively regulates genes involved in DNA repair pathways.
    • Rpn4 also modulates genes associated with the antioxidant response and glucose metabolism.

    Conclusions:

    • Rpn4 plays a multifaceted role in cellular defense against methyl methanesulfonate (MMS) induced stress.
    • The protective mechanism of Rpn4 extends beyond its known regulation of the ubiquitin-proteasome system (UPS).
    • Rpn4 coordinates the expression of genes critical for DNA repair, oxidative stress mitigation, and metabolic adaptation.

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