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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Immunoprecipitation01:20

Immunoprecipitation

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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
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Western Blotting01:15

Western Blotting

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Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
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[Methods of protein immunoanalysis].

N A Lisitsyn, A A Chernyĭ, I G Nikitina

    Molekuliarnaia Biologiia
    |April 7, 2015
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    Summary
    This summary is machine-generated.

    This review covers protein immunoanalysis methods like radioimmunoanalysis and ELISA. It details applications of these key immunoassay techniques for protein detection and quantification.

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    Area of Science:

    • Biochemistry and Molecular Biology
    • Immunology
    • Analytical Chemistry

    Context:

    • Protein analysis is crucial in diagnostics and research.
    • Accurate protein quantification requires sensitive and specific methods.
    • Various immunoassays have been developed to meet these needs.

    Purpose:

    • To review and compare current protein immunoanalysis techniques.
    • To highlight the applications of these methods.
    • To provide an overview of the field for researchers and practitioners.

    Summary:

    • Discusses radioimmunoanalysis (RIA), enzyme-linked immunosorbent assay (ELISA), immuno-PCR, electrochemical analysis, and chromatin immunoprecipitation (ChIP).
    • Details the principles and methodologies behind each technique.
    • Explores the diverse applications of these immunoassays in biological and medical research.

    Impact:

    • Provides a comprehensive overview of protein immunoanalysis tools.
    • Aids researchers in selecting appropriate methods for their specific applications.
    • Facilitates advancements in protein detection and biomarker discovery.