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[Reactivating factor of Luteococcus japonicus subsp. casei: isolation and characterization].

L I Vorob'eva, E A Rogozhin, E Iu Khodzhaev

    Prikladnaia Biokhimiia I Mikrobiologiia
    |April 7, 2015
    PubMed
    Summary

    A new subspecies, Luteococcus japonicus subsp. casei, produces reactivating factor (RF). This secreted polypeptide molecule exhibits antioxidant activity and acts as a signaling molecule, potentially offering protective effects.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Context:

    • A producer strain of reactivating factor (RF) was identified.
    • The strain is physiologically, biochemically, and genetically similar to Luteococcus japonicus DSM 10546.
    • Phenotypical differences led to its classification as a new subspecies, Luteococcus japonicus subsp. casei.

    Purpose:

    • To characterize the producer strain of reactivating factor (RF).
    • To investigate the nature and function of the secreted RF.
    • To identify bioactive metabolites produced by L. casei.

    Summary:

    • The producer strain was identified as Luteococcus japonicus subsp. casei, a subspecies of L. japonicus.
    • Reactivating factor (RF) is secreted into the medium and functions as a signaling molecule.
    • RF exhibits antioxidant activity, suggesting a protective role. Four biologically active components were isolated from the culture medium, with the most active being a polypeptide containing amino acid and sugar residues.

    Impact:

    • Establishes a new subspecies, Luteococcus japonicus subsp. casei.
    • Identifies RF as a secreted signaling molecule with potential therapeutic applications due to its antioxidant properties.
    • Characterizes novel bioactive metabolites from L. casei, opening avenues for further research in microbial products.