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Published on: April 25, 2015
Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription
Hyung-Ju Lim1, Kwangsoo Kim, Minsang Shin
1Department of Microbiology, Chonnam National University Medical School, Kwangju, 501-746, Republic of Korea.
Abstract:
σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone-like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The results suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.
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