Related Experiment Videos
Quantitative determination of pectic substances as an example of a rhamnopolysaccharide assay
P L Turecek1, E Buxbaum, F Pittner
1Institut für Allgemeine Biochemie der Universität Wien, Austria.
Journal of Biochemical and Biophysical Methods
|August 1, 1989
Abstract:
A quantitative enzymatic assay for rhamnopolysaccharides is described. The procedure is shown with pectic substances as an example. The test is based on the enzymatic degradation of the macromolecules to liberate L-rhamnose. This sugar can be quantitatively determined with the help of L-rhamnose dehydrogenase under concomitant reduction of NAD, thus allowing the quantitative evaluation of the original pectin.