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Improving cold storage and processing traits in potato through targeted gene knockout.

Benjamin M Clasen1, Thomas J Stoddard1, Song Luo1

  • 1Cellectis plant sciences Inc., New Brighton, MN, USA.

Plant Biotechnology Journal
|April 8, 2015
PubMed
Summary
This summary is machine-generated.

Transcription activator-like effector nucleases (TALENs) were used to knockout the vacuolar invertase gene (VInv) in potatoes. This significantly reduced reducing sugars, leading to lower acrylamide levels and lighter chip color in processed potatoes.

Keywords:
acrylamide reductioncold-induced sweeteninggene editingpotatotranscription activator-like effector nucleasesvacuolar invertase

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Area of Science:

  • Agricultural Science
  • Biotechnology
  • Food Science

Background:

  • Cold storage of potatoes increases reducing sugars, leading to undesirable browning and acrylamide formation during processing.
  • The vacuolar invertase gene (VInv) plays a key role in sucrose breakdown and subsequent sugar accumulation in potato tubers.

Purpose of the Study:

  • To utilize transcription activator-like effector nucleases (TALENs) for efficient gene knockout of VInv in a commercial potato variety.
  • To assess the impact of VInv knockout on reducing sugar levels and acrylamide formation in potato tubers.

Main Methods:

  • TALENs were employed to create mutations in the VInv gene in the Ranger Russet potato variety.
  • Gene editing efficiency was evaluated by analyzing VInv allele mutations and TALEN DNA integration.
  • Biochemical analysis of tubers and processed potato chips was conducted to measure sugar content and acrylamide levels.

Main Results:

  • Eighteen modified potato plant lines were generated, with five exhibiting complete VInv knockout.
  • Tubers from VInv-knockout plants showed undetectable levels of reducing sugars.
  • Processed potato chips from these tubers had reduced acrylamide content and lighter color.
  • Seven lines were free of TALEN DNA insertions, indicating successful gene editing without foreign DNA integration.

Conclusions:

  • TALENs offer a rapid and effective method for trait improvement in commercially relevant autotetraploid potato varieties.
  • Complete VInv knockout is a viable strategy to mitigate sugar accumulation and reduce acrylamide formation in potatoes.
  • This approach provides a promising framework for developing potatoes with enhanced processing quality and reduced health risks.