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Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions
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Layer by layer assembled films between hemoglobin and multiwall carbon nanotubes for pH-switchable biosensing.

Zhongqin Pan1, Xiaojun Liu1, Jing Xie2

  • 1Institute of Analytical Chemistry for Life Science, Nantong University, Nantong 226019, China; School of Public Health, Nantong University, Nantong 226019, China.

Colloids and Surfaces. B, Biointerfaces
|April 8, 2015
PubMed
Summary

This study presents a pH-switchable biosensor for hydrogen peroxide (H2O2) detection using hemoglobin (Hb) and multiwall carbon nanotubes (MWCNTs). The novel sensor demonstrates tunable sensitivity based on solution pH, overcoming limitations of previous designs.

Keywords:
BiosensingHemoglobinLayer by layer assemblyMultiwall carbon nanotubespH switchable

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Area of Science:

  • Electrochemistry
  • Biosensing
  • Materials Science

Background:

  • pH-switchable behaviors in modified electrodes are known, but pH-switchable biosensing remains challenging due to electroactive mediators.
  • Existing biosensors often struggle with precise control over pH-dependent detection.

Purpose of the Study:

  • To develop a pH-switchable biosensor for hydrogen peroxide (H2O2) determination.
  • To investigate the influence of pH on the performance of a multilayer film-modified electrode.

Main Methods:

  • Fabrication of a four-bilayer film using layer-by-layer assembly of hemoglobin (Hb) and multiwall carbon nanotubes (MWCNTs).
  • Electrochemical characterization using cyclic voltammetry with an electroactive probe (Fe(CN)6(3-)).
  • Determination of H2O2 using the modified electrode at different pH values.

Main Results:

  • The multilayer film exhibited sensitive and reversible responses to pH changes in phosphate buffer solutions.
  • The reduction peak height of Fe(CN)6(3-) varied significantly with pH, from ~221μA at pH 3.0 to 0μA at pH 9.0.
  • A wide linear detection range for H2O2 (12.5μM to 10.4mM) was achieved at pH 3.0.

Conclusions:

  • The developed modified electrode shows pH-dependent determination of H2O2.
  • The composition of multilayer films impacts the pH-switchable biosensing performance.
  • This work offers a promising approach for tunable biosensing applications.