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Open source software for quantification of cell migration, protrusions, and fluorescence intensities.

David J Barry1, Charlotte H Durkin1, Jasmine V Abella1

  • 1The Francis Crick Institute, Lincoln's Inn Fields Laboratories, London WC2A 3LY, England, UK.

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Summary

This study introduces ADAPT, an automated ImageJ plugin for quantifying cell migration and morphodynamics. It offers a user-friendly solution for analyzing cell movement and membrane structures, overcoming limitations of manual methods.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Computational Biology

Background:

  • Cell migration involves dynamic changes in cell shape (morphodynamics).
  • Manual analysis of cell morphodynamics, such as using kymographs, is time-consuming and prone to bias.
  • Automated, user-friendly tools for quantitative morphodynamic analysis are lacking.

Purpose of the Study:

  • To develop and present ADAPT, an automated software tool for quantifying cell migration and morphodynamics.
  • To provide a user-friendly, open-source solution for researchers studying cell movement.
  • To enable unbiased, high-throughput analysis of cellular morphodynamics.

Main Methods:

  • Development of ADAPT as an ImageJ plugin.
  • Automated quantification of cell migration, membrane protrusions, and associated proteins.
  • Application of ADAPT to analyze cell migration on various extracellular matrices.
  • Morphological profiling of filopodia and blebs using ADAPT.
  • Quantification of Arp2/3 complex inhibition effects on bleb retraction.

Main Results:

  • ADAPT enables rapid, automated analysis of cell migration and morphodynamics across multiple cells.
  • The software successfully quantified variations in cell migration rates on different extracellular matrices.
  • ADAPT can accurately detect and morphologically profile filopodia.
  • An unbiased description of bleb formation and retraction dynamics was generated using ADAPT.
  • The effect of Arp2/3 complex inhibition on bleb retraction was quantitatively assessed.

Conclusions:

  • ADAPT provides a powerful, automated solution for the quantitative analysis of cell migration and morphodynamics.
  • The software facilitates unbiased, high-throughput research in cell motility and membrane dynamics.
  • ADAPT overcomes limitations of manual analysis, making advanced morphodynamic studies more accessible.