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Updated: Apr 15, 2026

Visualization of G3BP Stress Granules Dynamics in Live Primary Cells
Published on: May 21, 2014
G3BP1 promotes stress-induced RNA granule interactions to preserve polyadenylated mRNA
Anaïs Aulas1, Guillaume Caron1, Christos G Gkogkas2
1Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada Centre de recherché du Centre Hospitalier de l'Université de Montréal (CRCHUM), Department of Biochemistry, and Department of Neuroscience, Université de Montréal, Montréal, QC, H2X 0A9, Canada.
Abstract:
G3BP1, a target of TDP-43, is required for normal stress granule (SG) assembly, but the functional consequences of failed SG assembly remain unknown. Here, using both transformed cell lines and primary neurons, we investigated the functional impact of this disruption in SG dynamics. While stress-induced translational repression and recruitment of key SG proteins was undisturbed, depletion of G3BP1 or its upstream regulator TDP-43 disturbed normal interactions between SGs and processing bodies (PBs). This was concomitant with decreased SG size, reduced SG-PB docking, and impaired preservation of polyadenylated mRNA. Reintroduction of G3BP1 alone was sufficient to rescue all of these phenotypes, indicating that G3BP1 is essential for normal SG-PB interactions and SG function.
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