[Immunoanalytical profile of free tri-iodothyronine]

Radhouene Doggui1, Jacques Ingrand2

  • 1Laboratoire de surveillance et épidémiologie nutritionnelles en Tunisie (Surven), Institut national de nutrition et de technologie alimentaire, Tunis, Tunisie.

Tri-iodothyronine (T3) is the active form of thyroid hormone at the nuclear cell level. About 80% of tri-iodothyronine is produced by peripheral deiodination of thyroxine. The determination of the free form of this hormone is useful for the diagnosis and the follow-up of hyperthyroidism. In routine clinical laboratories, immunoassays remain the most used technics. After recalling the main points related to the physiological data, the authors point out the possible errors during the analytical steps of the assay.

Related Concept Videos

Synthesis and Regulation of Thyroid Hormones01:20

Synthesis and Regulation of Thyroid Hormones

Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
9.4K
Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
319
Redox Titration: Iodimetry and Iodometry01:23

Redox Titration: Iodimetry and Iodometry

Iodometry and iodimetry are analytical methods used to determine the concentration of oxidizing or reducing agents using iodine. In iodometric titrations, the oxidizing analyte solution is usually acidified and treated with an excess of iodide ions, which generates an equivalent amount of iodine in equilibrium with triiodide. The released iodine is subsequently titrated directly against a standardized reducing agent. As the dilute iodine color becomes pale yellow, a few drops of freshly...
7.4K