Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles
Priyanka Singh1, Yeon Ju Kim2, Hina Singh1
1Department of Oriental Medicinal Material and Processing, Kyung Hee University, Yongin, Republic of Korea.
International Journal of Nanomedicine
|April 8, 2015
Summary
Brevibacterium frigoritolerans DC2 efficiently synthesized silver nanoparticles (AgNPs). These AgNPs showed antimicrobial activity and enhanced the efficacy of common antibiotics against various pathogens.
Area of Science:
- Microbiology
- Nanotechnology
- Materials Science
Background:
- Bacterial strains offer a sustainable platform for nanoparticle biosynthesis.
- Silver nanoparticles (AgNPs) exhibit significant antimicrobial properties.
- Enhancing antibiotic efficacy is crucial for combating resistant pathogens.
Purpose of the Study:
- To explore Brevibacterium frigoritolerans DC2 for extracellular silver nanoparticle synthesis.
- To characterize the biosynthesized silver nanoparticles.
- To evaluate the antimicrobial activity of AgNPs and their synergistic effects with antibiotics.
Main Methods:
- Extracellular biosynthesis of silver nanoparticles using Brevibacterium frigoritolerans DC2.
- Characterization via UV-Vis spectrophotometry, FESEM, DLS, EDS, and XRD.
- Antimicrobial susceptibility testing against pathogenic bacteria and fungi.
- Evaluation of combined effects with commercial antibiotics.
Main Results:
- Efficient synthesis of spherical silver nanoparticles (average size 97 nm) confirmed by characterization techniques.
- Demonstrated broad-spectrum antimicrobial activity against Vibrio parahaemolyticus, Salmonella enterica, Bacillus anthracis, Bacillus cereus, Escherichia coli, and Candida albicans.
- Significant enhancement of antibiotic efficacy when combined with biosynthesized silver nanoparticles.
Conclusions:
- Brevibacterium frigoritolerans DC2 is a viable agent for eco-friendly silver nanoparticle production.
- Biosynthesized silver nanoparticles possess inherent antimicrobial capabilities.
- Co-administration of silver nanoparticles potentiates the antimicrobial action of conventional antibiotics, offering a promising strategy for infectious disease treatment.
