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[Experimental studies on haematogenous osteomyelitis due to Staphylococcus hyicus in mice]
1Department of Orthopaedic Surgery, St. Marianna University School of Medicine, Kanagawa, Japan.
Abstract:
10(7) colony forming units of 18 different strains of Staphylococcus hyicus were intravenously administered into mice. Five mice as a group received each strain of S. hyicus. At 14 days after inoculation, 11 out of 18 strains were found in the bone marrow. Out of these 11 strains, SMUH 10 and SMUH 18 caused remarkable inflammation which was characterized by roentgenographical change. A number of these organisms were detected in the bone marrow until 28 and 42 days after the injection, suggesting specific lodgement of the strain in mouse bone marrow. X-rays of the upper and lower extremities taken 14 days after inoculation showed multiple small radiolucent areas surrounded by small rims representing osteosclerosis. However, these changes did not show any aggravation during 70 days after inoculation suggesting tendency of improvement.
Insights
Certain Staphylococcus hyicus strains can lodge in mouse bone marrow, causing inflammation and bone changes. Some strains showed specific bone marrow tropism and induced osteosclerosis, with signs of improvement over time.
Area of Science:
- Microbiology
- Immunology
- Veterinary Pathology
Context:
- Bacterial infections can affect bone health.
- Staphylococcus hyicus is a known pathogen in animals.
- Understanding bacterial tropism is crucial for disease management.
Purpose:
- To investigate the bone marrow tropism of Staphylococcus hyicus strains.
- To characterize the pathological changes induced by S. hyicus in mouse bone.
- To assess the temporal dynamics of bacterial presence and bone lesions.
Summary:
- Intravenous administration of 18 Staphylococcus hyicus strains to mice revealed that 11 strains successfully colonized the bone marrow.
- Two strains, SMUH 10 and SMUH 18, induced significant bone inflammation, evidenced by roentgenographical changes.
- Radiolucent areas and osteosclerosis were observed in extremities, with a tendency towards improvement by 70 days post-inoculation.
Impact:
- This study highlights the potential of S. hyicus to cause osteomyelitis.
- Identifies specific strains with bone-homing capabilities.
- Provides insights into the pathogenesis of Staphylococcus-induced bone disease.