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MicroRNA399 is involved in multiple nutrient starvation responses in rice

Bin Hu1, Wei Wang2, Kun Deng3

  • 1State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing, China.

Frontiers in Plant Science
|April 9, 2015
PubMed

Insights

MicroRNAs (miRNAs) regulate nutrient stress. In rice, OsmiR399 controls phosphorus, iron, potassium, sodium, and calcium absorption, revealing complex nutrient interactions.

Area of Science:

  • Plant Molecular Biology
  • Nutrient Homeostasis
  • MicroRNA Regulation

Background:

  • MicroRNAs (miRNAs) are key regulators of nutrient stress responses.
  • miR399 is known to be induced by phosphorus (P) starvation.
  • The precise role of miRNAs in multi-nutrient interactions remains to be fully elucidated.

Purpose of the Study:

  • To investigate the function of OsmiR399 in rice (Oryza sativa L.) beyond phosphorus starvation.
  • To explore the role of OsmiR399 in the response to other nutrient stresses.
  • To understand the interaction among different nutrients mediated by OsmiR399.

Main Methods:

  • GeneChip analysis of OsmiR399 over-expressing rice plants.
  • Measurement of nutrient concentrations (Fe, K, Na, Ca) in plant tissues.
  • Analysis of OsmiR399 expression under various nutrient starvation conditions.
  • Loss-of-function studies of OsmiR399's downstream target, LTN1.

Main Results:

  • Over-expression of OsmiR399 up-regulated genes involved in iron (Fe), potassium (K), sodium (Na), and calcium (Ca) absorption.
  • Increased concentrations of Fe, K, Na, and Ca were observed in OsmiR399 over-expressing plants.
  • OsmiR399 expression was induced by starvation of Fe, K, Na, and Ca.
  • Loss-of-function of LTN1 mimicked the effects of OsmiR399 over-expression, increasing multiple mineral elements.

Conclusions:

  • OsmiR399 plays a significant role in regulating responses to multiple nutrient starvations in rice.
  • OsmiR399 mediates interactions between different nutrient elements.
  • This study provides a new perspective on miRNA-mediated nutrient crosstalk.
Keywords:
gene expressionmicroRNA399 (miR399)nutrient starvation responseover-expressing plantsrice

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