Design of an osteoinductive extracellular fibronectin matrix protein for bone tissue engineering
Sujin Lee1, Dong-Sung Lee2, Ilsan Choi3
1Department of Biochemistry, College of Medicine, Inha University, Incheon 400-712, Korea. sujin2manse@naver.com.
Abstract:
Integrin-mediated cell-matrix interactions play an important role in osteogenesis. Here, we constructed a novel osteoinductive fibronectin matrix protein (oFN) for bone tissue engineering, designed to combine the integrin-binding modules from fibronectin (iFN) and a strong osteoinductive growth factor, bone morphogenetic protein-2. Compared with iFN, the purified oFN matrix protein caused a significant increase in cell adhesion and osteogenic differentiation of pre-osteoblast MC3T3-E1 cells (p < 0.05).
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