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Updated: Apr 15, 2026

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
Published on: January 17, 2019
A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules
Kento Yamagishi1, Kazuaki Sawaki, Atsushi Murata
1Department of Life Science & Medical Bioscience, Graduate School of Advanced Science & Engineering, Waseda University, TWIns 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan. takeoka@waseda.jp.
Abstract:
We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.
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