Related Experiment Video
Updated: Apr 15, 2026

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis
Published on: March 28, 2025
Distribution and function of prophage phiRv1 and phiRv2 among Mycobacterium tuberculosis complex
Xiangyu Fan1,2, Abu Algasim Elgaili Abd Alla1, Jianping Xie1
1a State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, Institute of Modern Biopharmaceuticals, School of Life Sciences , Southwest University , Beibei, Chongqing 400700 , China.
Abstract:
Mycobacterium tuberculosis complex (MTBC) is notorious for causing diseases, such as tuberculosis. Tuberculosis caused by M. tuberculosis remains a global public health concern. Two prophages, phiRv1 and phiRv2, can be found among most MTBC genomes. However, no precise functions have been assigned for the two prophages. In this paper, to find out the function of these two prophages, the distribution and function of phiRv1 and phiRv2 in MTBC genomes were analyzed from multiple omics data. We found that complex insertion, deletion, and reorganization appeared on the locus of two prophages in MTBC genomes; some genes of the two prophages can be translated and are functional from proteomic data; the expression of other prophage genes, such as Rv1577c, Rv2650c, Rv2652c, Rv2659c, and Rv2658c, can vary with environmental stresses and might enhance the fitness of MTBC. These data will facilitate our in-depth understanding of their function.
Related Concept Videos
DNA Bacteriophages
Lysogenic Cycle of Bacteriophages
Bacterial Phylum Verrucomicrobiota
Viral Replication: Lysogenic Cycle
Lytic Cycle of Bacteriophages
Regulation of Bacterial Virulence

