Related Experiment Video
Updated: Apr 15, 2026
07:54
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
Published on: July 25, 2017
14.9K
Lactic Acid Bacteria and Bifidobacteria-Inhibited Staphylococcus epidermidis
Amy-Sie-Yik Lau1, Min-Tze Liong2
1School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia.
Wounds : a Compendium of Clinical Research and Practice
|April 10, 2015
Summary
Lactic acid bacteria and bifidobacteria produce antimicrobial compounds effective against Staphylococcus epidermidis, an opportunistic skin pathogen. These natural compounds, including organic acids and hydrogen peroxide, show significant inhibitory activity in wound infections.
Area of Science:
- Microbiology
- Biotechnology
- Dermatology
Background:
- Lactic acid bacteria (LAB) and bifidobacteria synthesize antimicrobial substances like organic acids, bacteriocins, hydrogen peroxide, and diacetyl.
- Staphylococcus epidermidis is a significant opportunistic pathogen in wound infections, particularly those associated with medical implants.
Purpose of the Study:
- To screen LAB and bifidobacteria strains for antimicrobial activity against Staphylococcus epidermidis.
- To identify and quantify the specific antimicrobial compounds produced by effective LAB strains.
Main Methods:
- Spectrophotometric method using a 96-well microplate reader to assess inhibitory activity.
- Analytical methods were employed to characterize the cell-free supernatant (CFS).
- Selected strains with high inhibition percentages (73.7%-88.2%) were analyzed further.
Main Results:
- Lactic acid was the predominant organic acid produced by all tested strains.
- Lactobacillus fermentum BD 1912d produced the highest concentration of hydrogen peroxide (0.014 mg/mL).
- Weissella cibaria FTDC 8643 exhibited the highest diacetyl production (2.884 ng/mL).
Conclusions:
- Antimicrobial metabolites from LAB's CFS effectively inhibited the growth of Staphylococcus epidermidis.
- The study highlights the potential of LAB-derived compounds in managing opportunistic skin pathogens.
More Related Videos
07:42Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition
Published on: September 3, 2016
15.7K
06:36Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds
Published on: September 8, 2021
3.4K
Related Concept Videos
01:25Clinical Significance of Antibiotic Resistance
57
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
57
01:27Microbes in the Production of Fermented Foods
248
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
248
01:27Bacterial Phylum Firmicutes
1.3K
Firmicutes is a diverse phylum of Gram-positive bacteria characterized by a low GC content in their genomes. This phylum includes organisms with monoderm or diderm cell envelopes, highlighting a complex evolutionary history. Firmicutes comprises several major orders, including Lactobacillales, Clostridiales, and Bacillales, which exhibit remarkable diversity in their morphology, metabolism, and ecological roles.The order Lactobacillales includes lactic acid bacteria, which are fermentative...
1.3K
01:27The Skin Microbiota
83
The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
83
01:29Staphylococcal Skin Infections
99
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
99
01:30Bacterial Phylum Actinobacteria
882
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
882