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Comparative genome analyses of Serratia marcescens FS14 reveals its high antagonistic potential

Pengpeng Li1, Amy H Y Kwok1, Jingwei Jiang2

  • 1Bioinformatics Center, Nanjing Agricultural University, Nanjing, China.

Plos One
|April 10, 2015
PubMed

Insights

Serratia marcescens FS14, isolated from a diseased plant, underwent genome sequencing. Comparative genomics revealed its antagonistic mechanisms against plant pathogens, including unique secretion systems and antibiotic resistance.

Area of Science:

  • Genomics
  • Microbiology
  • Plant Pathology

Background:

  • Serratia marcescens FS14 was isolated from Atractylodes macrocephala Koidz exhibiting root rot symptoms, linked to Fusarium oxysporum infection.
  • The study addresses the need for a comprehensive genomic understanding of the Serratia genus, particularly regarding plant-associated strains.

Purpose of the Study:

  • To perform the first comprehensive comparative-genomic analysis of the Serratia genus.
  • To identify genetic mechanisms underlying the antagonism of S. marcescens FS14 against phytopathogens.
  • To investigate the diversity and function of Type VI secretion systems (T6SSs) within the Serratia genus.

Main Methods:

  • Whole-genome sequencing of S. marcescens FS14.
  • Pan-genome and Clusters of Orthologous Groups (COG) analyses for conserved and diverse genes.
  • Comparative genomic analysis focusing on specific systems (e.g., Type I restriction-modification, Type III secretion, T6SSs) and resistance determinants.
  • Competition bioassays against Ralstonia solanacearum, Fusarium oxysporum, and Sclerotinia sclerotiorum.

Main Results:

  • Comparative genomics revealed conserved core genes for basic functions and significant genome diversity driven by factors like prophages.
  • S. marcescens FS14 possesses multiple antagonistic traits, including prodigiosin, bacteriocins, chitinases, and multi-antibiotic resistance.
  • FS14 harbors two distinct Type VI secretion systems (T6SSs), contributing to its competitive advantage; four types of T6SSs were identified across Serratia species.
  • Bioassays confirmed high antagonistic activity of FS14 against R. solanacearum, F. oxysporum, and S. sclerotiorum.

Conclusions:

  • The genome of S. marcescens FS14 provides insights into its adaptation and competitive strategies in plant environments.
  • Comparative genomics highlights the diversity within the Serratia genus, particularly concerning virulence and defense mechanisms like T6SSs.
  • S. marcescens FS14 demonstrates significant potential as a biocontrol agent against major bacterial and fungal plant pathogens.

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