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Updated: Apr 15, 2026
A Mouse Model of Lumbar Spine Instability
Published on: April 23, 2021
Paraspinal Muscle Spindle Response to Intervertebral Fixation and Segmental Thrust Level During Spinal Manipulation
William R Reed1, Joel G Pickar
1From the Palmer Center for Chiropractic Research, 741 Brady Street, Davenport, IA.
Study Design:
In vivo cat model study.
Objective:
To determine whether intervertebral facet joint fixation and segmental thrust level alter paraspinal muscle spindle activity during simulated spinal manipulation.
Summary Of Background Data:
Intervertebral motion is commonly assessed by manual therapy practitioners during clinical evaluation and treatment. Mechanoreceptor activity elicited during spinal manipulation has been theorized as a potential mechanism of its efficacy. The degree to which intervertebral fixation and segmental thrust level alter paraspinal muscle spindle activity during high velocity low amplitude spinal manipulation (HVLA-SM) is unclear.
Methods:
Intervertebral fixation was created by inserting facet screws through the left L(5-6) and L(6-7) and left L(4-5), L(5-6), and L(6-7) facet joints of a cat spine. Changes in the mean instantaneous frequency of L6 muscle spindle discharge were determined during 5 HVLA-SM thrust durations (0-control, 75, 100, 150, 250 ms) delivered at the L4 or L6 spinous process in each of the 3 conditions within the same preparation: laminectomy-only (surgical control; n = 23), L(5-6) and L(6-7) fixations (n = 20), and L(4-5), L(5-6), and L(6-7) fixations (n = 7). Comparisons were made between thrust levels, thrust durations, and spinal joint conditions using a linear mixed model.
Results:
Insertion of facet screws compared with laminectomy-only significantly increased (P < 0.001) lumbar spinal stiffness during L6 HVLA-SM. Compared with laminectomy-only, both the 2 facet screw (100 ms; P < 0.05) and 3 screw conditions [75 ms and 100 ms (P < 0.001), 150 ms (P < 0.005), and 250 ms (P < 0.05)] significantly decreased L6 spindle response during the L6 HVLA-SM. HVLA-SM-delivered 2 segments rostral to the level of muscle spindle input significantly decreases spindle response compared with HVLA-SM-delivered at-level; however, nontarget HVLA-SM still elicits 60% to 80% of at-level muscle spindle response.
Conclusion:
Intervertebral fixation decreases paraspinal muscle spindle response during L6 HVLA-SM in a cat model. Whereas HVLA-SM target accuracy maximizes spindle response, nontarget HVLA-SM still elicits substantial levels of muscle spindle activity.
Level Of Evidence:
N/A.
