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Updated: Apr 15, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Characterization of the mitochondrial genome of Bufo gargarizans minshanicus (Anura: Bufonidae)
Jiandong Yang1, Jiabin Liu1, Rui Xue1
1a College of Animal Science and Technology, Sichuan Agricultural University , Chengdu , China and.
Abstract:
The complete mitogenome of Bufo gargarizans minshanicus is 17,719 bp in size, and shares a conserved gene arrangement, gene content and base composition with other Bufonidae species. The base composition of the H-strand is biased toward A + T content at 56.97% (T 28.14%, C 27.67%, A 28.83% and G 15.36%). Most protein-coding genes (PCGs) begin with ATG, while COX1 and ND2 initiate with ATA and ND1 starts at ATT. Seven PCGs have the complete stop codons AGA and TAA, whereas the remaining four PCGs end with TA or T. The putative control region possesses two repeat motifs, and four termination-associated sequences and six conserved sequence blocks at the 5' and 3' ends.
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