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Updated: Apr 15, 2026
Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
[The effect of platelet-rich fibrin on biologic characteristics of osteoblasts]
Xiao-Lin Sun1, Yan-Min Zhou, Jing-Hui Zhao
1Department of Oral implantation, Jilin University Affiliated Stomatology Hospital. Changchun 130021, Jilin Province, China.E-mail:sxl2673366@126.com.
Purpose:
To investigate the effect of platelet-rich fibrin on biologic characteristics of osteoblasts.
Methods:
Osteoblasts were cultured with or without a PRF membrane. The cell proliferation was detected by MTT. The alkaline phosphatase (ALP) stain and the positive expression of collagen type I, osteoprotegerin and RANKL were measured. The data was statistically analyzed with SPSS17.0 software package.
Results:
PRF promoted cell proliferation and increased expression of ALP, collagen typeI and OPG significantly, but there was no significant impact on expression of RANKL.
Conclusions:
PRF promotes osteoblasts proliferation, differentiation and OPG expression. PRF is involved in bone remodeling via regulating expression of OPG and RANKL.
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