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Comparison of the CT OligoGen kit with cobas 4800 assay for detection of Chlamydia trachomatis
Manuel Parra-Sánchez1, Ana Marcuello-López2, Silvia García-Rey1
1Unit of Infectious Disease and Clinical Microbiology, Valme University Hospital, Seville, Spain.
Introduction:
A prospective study was designed to assess the performance of the new CT OligoGen kit and the cobas 4800 assay for detection of Chlamydia trachomatis.
Methods:
A set of samples that included urine samples (n=212), endocervical (n=167), rectal (n=53), pharyngeal (n=7) and urethral swabs (n=3). The samples were sent from a regional sexually transmitted diseases (STD) clinic in Seville, Spain, and were collected from 261 men and 181 women. Discordant results were re-analyzed and clinical data and other tests were reviewed in order to resolve them.
Results:
Sensitivity, specificity, positive predicative value (PPV), negative predictive value (NPV) and kappa value for C. trachomatis detection using the CT OligoGen kit were 98.5%, 100%, 100%, 95.4% and 0.97, respectively.
Conclusions:
This new kit had a high sensitivity, specificity, PPV and NPV for C. trachomatis, therefore the performance profile confirms the usefulness and reliable results of this new assay.
