Related Experiment Video

Updated: Apr 15, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.5K

Emergence of multidrug resistant enterococci at a tertiary care centre

Puneet Bhatt1, Anubha Patel2, A K Sahni3

  • 1Graded Specialist (Microbiology), Command Hospital (Southern Command), Pune 411040, India.

Medical Journal, Armed Forces India
|April 11, 2015
PubMed
Abstract

Insights

Multidrug resistant enterococci are a growing concern, with 63% of isolates showing resistance. High-level aminoglycoside resistance and glycopeptide resistance were prevalent, posing a significant therapeutic challenge.

Area of Science:

  • Clinical microbiology
  • Infectious diseases
  • Antimicrobial resistance

Background:

  • Enterococci are clinically significant due to increasing antimicrobial resistance.
  • Understanding antibiograms of multidrug-resistant (MDR) enterococci is crucial for effective antibiotic policies.
  • This study aimed to assess antimicrobial resistance patterns and MDR prevalence in enterococci.

Purpose of the Study:

  • To determine the prevalence of multidrug resistance in clinical enterococcal isolates.
  • To analyze the antimicrobial resistance patterns of these isolates.
  • To identify specific resistance trends, including high-level aminoglycoside resistance (HLAR) and glycopeptide resistance.

Main Methods:

  • A cross-sectional study involving 200 non-repetitive clinical enterococcal isolates.
  • Antimicrobial susceptibility testing performed using the disc diffusion method.
  • Minimum Inhibitory Concentration (MIC) determined for key antibiotics (gentamicin, streptomycin, vancomycin, teicoplanin, linezolid) via E-test.

Main Results:

  • A high prevalence of multidrug resistance (MDR) was observed in 63% of enterococcal isolates.
  • Most isolates exhibited resistance to penicillin (95%), ampicillin (95%), and cotrimoxazole (90%).
  • High-level aminoglycoside resistance (HLAR) was found in 39% and glycopeptide resistance in 14% of isolates. Resistance to linezolid was low (2%).

Conclusions:

  • The study confirms a 63% prevalence of MDR enterococci, with higher rates in Enterococcus faecium compared to Enterococcus faecalis.
  • Emergence and increased prevalence of MDR enterococci present a significant therapeutic challenge.
  • Findings underscore the need for continuous monitoring and updated antibiotic strategies against resistant enterococcal infections.
Keywords:
Antimicrobial susceptibility testingGlycopeptide resistanceHigh-level aminoglycoside resistanceMinimum inhibitory concentrationMultidrug resistant enterococci

More Related Videos

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

12.6K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.1K

Related Concept Videos

Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
141
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
57
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
2.0K
Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...
62
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care01:30

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...
411
Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic01:26

Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic

Healthcare-associated infections (HAIs) occur in a healthcare facility while a person receives care for another ailment. This category also includes work-related infections among healthcare staff.
HAIs significantly increase the cost of health care. Extended stays in healthcare institutions, increased disability, increased costs of medications, including specialized antibiotics, and prolonged recovery times add to the patient's expenses and the healthcare institution and funding bodies.
6.7K