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Updated: Apr 15, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
Published on: August 2, 2015
Hexahistidine (6xHis) fusion-based assays for protein-protein interactions
1Department of Pharmacology, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA, 30322, USA, marycpuckett@gmail.com.
Abstract:
Fusion-protein tags provide a useful method to study protein-protein interactions. One widely used fusion tag is hexahistidine (6xHis). This tag has unique advantages over others due to its small size and the relatively low abundance of naturally occurring consecutive histidine repeats. 6xHis tags can interact with immobilized metal cations to provide for the capture of proteins and protein complexes of interest. In this chapter, a description of the benefits and uses of 6xHis-fusion proteins as well as a detailed method for performing a 6xHis-pulldown assay are described.
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