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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Related Experiment Video

Updated: Apr 15, 2026

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Published on: November 20, 2017

Split-luciferase complementation assay to detect channel-protein interactions in live cells.

Alexander S Shavkunov¹, Syed R Ali, Neli I Panova-Elektronova

¹Department of Pharmacology and Toxicology, The University of Texas Medical Branch, Medical Research Building, 301 University Boulevard, Galveston, TX, 77555, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 11, 2015

Summary

Researchers developed a novel split-luciferase assay to rapidly study protein interactions with voltage-gated sodium channels. This method aids in understanding ion channel function and discovering new drug targets.

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Area of Science:

Molecular Pharmacology
Cellular Biology
Biophysics

Background:

Protein-protein interactions are crucial for ion channel function and represent key drug targets.
Current methods for studying ion channel complexes are often slow and technically demanding.
There is a need for faster, more accurate techniques to investigate these interactions.

Purpose of the Study:

To introduce a new, rapid application of the split-luciferase complementation assay.
To investigate the interaction between voltage-gated sodium channels and fibroblast growth factor 14.
To explore the dynamic regulation of these interactions in live cells.

Main Methods:

Application of a split-luciferase complementation assay.
Studying protein-protein interactions in live cells.
Focusing on voltage-gated sodium channels and fibroblast growth factor 14.

Main Results:

Demonstrated a novel split-luciferase assay for studying ion channel complexes.
Successfully investigated the interaction between voltage-gated Na+ channels and fibroblast growth factor 14.
Showcased the dynamic regulation of these interactions in real-time.

Conclusions:

The split-luciferase assay offers a flexible and accessible tool for ion channel research.
This method can complement existing structural and functional studies.
It provides a foundation for new drug discovery strategies targeting protein-channel interactions.