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A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood
Published on: August 15, 2025
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A cost-effective method for obtaining standard platelet-rich plasma.
Yavuz Kececi1, Sibel Ozsu2, Oktay Bilgir2
1Izmir Education and Research Hospital, Plastic and Reconstructive Surgery, Ismir, Turkey; emai: yavuz.kececi@gmail.com.
Wounds : a Compendium of Clinical Research and Practice
|April 11, 2015
Summary
Standardizing platelet-rich plasma (PRP) preparation is crucial for consistent healing outcomes. This study found that adjusting centrifugation force based on individual blood characteristics can achieve a standard platelet concentration, optimizing PRP efficacy.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Surgical Innovation
Background:
- Platelet-rich plasma (PRP) enhances surgical healing by concentrating autologous platelets.
- Variability in PRP preparation protocols contributes to inconsistent clinical outcomes.
- Standardization of PRP preparation is needed to optimize therapeutic benefits.
Purpose of the Study:
- To develop a standardized protocol for preparing platelet-rich plasma (PRP).
- To investigate the effect of varying centrifugation forces on platelet concentration.
- To establish a method for achieving consistent PRP yields.
Main Methods:
- Whole blood samples were collected from 18 healthy donors.
- A double centrifugation protocol was employed with varying forces (300 g to 2000 g).
- Platelet counts and concentration factors were statistically analyzed.
Main Results:
- Significant increases in platelet counts were observed with increasing centrifugation force.
- Optimal differentiation in platelet concentration occurred between 500 g and 1000 g.
- Platelet concentration factors ranged from 1.92-fold to 3.76-fold.
Conclusions:
- Adjusting centrifugation force can standardize platelet concentration in PRP.
- Individualized adjustments based on baseline values may yield consistent PRP.
- This approach addresses variability in PRP preparation for enhanced clinical application.
