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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
Published on: May 28, 2021
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Quantitative proteogenomic profiling of epidermal barrier formation in vitro
Jason M Winget1, Julian D Watts1, Michael R Hoopmann1
1Institute for Systems Biology, 401 Terry Ave N., Seattle, WA 98109, USA.
Journal of Dermatological Science
|April 12, 2015
Summary
This study reveals over 3600 proteins during epidermal development, with 1200 changing abundance. Proteomic analysis is crucial for understanding skin barrier formation and maturation.
Area of Science:
- Biochemistry
- Proteomics
- Dermatology
Background:
- The skin barrier is vital for health, but its development is poorly understood.
- Epidermal barrier defects link to various skin diseases.
- System-level proteomic changes during skin development are largely uncharacterized.
Purpose of the Study:
- To create a quantitative proteogenomic profile of in vitro reconstituted epidermis.
- To characterize protein abundance changes during epidermal stratification and development.
Main Methods:
- Utilized data-dependent "shotgun" mass spectrometry for proteomic analysis.
- Employed statistically validated label-free quantification.
- Analyzed 20 replicates across three developmental time points.
Main Results:
- Identified over 3600 proteins in reconstituted epidermis.
- Observed abundance changes in more than 1200 proteins over the developmental time course.
- Collected matched transcriptomic data for comparison with proteomic findings.
Conclusions:
- Presents the most comprehensive epidermal proteome to date.
- Provides insights into skin barrier formation and composition.
- Highlights the necessity of proteomic analysis, as transcript and protein levels show limited correlation during epidermal maturation.
