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Updated: Apr 15, 2026

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
07:55

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

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Mutants of Schizosaccharomyces pombe which sporulate in the haploid state

Y Lino, M Yamamoto

    Molecular & General Genetics : MGG
    |April 14, 2015
    PubMed

    Insights

    Suppressor mutations in Schizosaccharomyces pombe enable haploid cells to sporulate, bypassing normal meiosis requirements. These mutations, defining the pat1 gene, cause temperature sensitivity and affect spore viability.

    Area of Science:

    • * Molecular and Cellular Biology
    • * Yeast Genetics
    • * Reproductive Biology

    Background:

    • * Meiosis is a critical cell division process for sexual reproduction, typically requiring specific genetic and environmental conditions.
    • * Mutations in mating type genes, such as mei1-102 in Schizosaccharomyces pombe, can block meiotic progression.
    • * Identifying suppressor mutations can reveal novel regulatory pathways in meiosis.

    Purpose of the Study:

    • * To isolate and characterize suppressor mutations of mei1-102 in Schizosaccharomyces pombe.
    • * To investigate the genetic basis and phenotypic consequences of these suppressors.
    • * To explore the potential for inducing sporulation under non-standard conditions.

    Main Methods:

    • * Isolation and characterization of suppressor mutants of mei1-102.
    • * Genetic analysis to confirm haploidy and identify the suppressor gene.
    • * Measurement of cellular DNA content.
    • * Phenotypic analysis of growth and sporulation at restrictive temperatures.

    Main Results:

    • * Suppressor mutations conferred temperature- or cold-sensitivity, halting growth and initiating sporulation at restrictive temperatures.
    • * Mutants sporulated under conditions not usually permitting meiosis, including in the presence of a nitrogen source and from a haploid state.
    • * The recessive suppressor mutations defined a single gene, pat1, located near the chromosome II centromere.
    • * A mei5 mutation suppressed the temperature sensitivity of pat1 mutants, indicating gene interaction.

    Conclusions:

    • * The pat1 gene plays a crucial role in regulating the initiation of meiosis.
    • * Haploid sporulation is achievable through specific genetic alterations, challenging conventional understanding of meiosis.
    • * The identified suppressor mutations provide new tools for studying meiotic regulation in yeast.

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