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Updated: Apr 15, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis
Cheng-Kuo Lai1, Jing-Chi Su, Yu-Hsin Lin
1School of Medicine & Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan.
Aim:
The aim of this study was to investigate whether cholesterol plays a pivotal role in cytolethal distending toxin (CDT) mediated pathogenic effects in hosts.
Materials & Methods:
The molecular mechanisms underlying cholesterol sequestering conferred resistance to CDT-induced DNA double-strand breaks (DSBs) and cell cycle arrest were investigated. Histopathological analysis was conducted for evaluating CDT-induced intestinal inflammation in mouse.
Results:
CDT actions were attenuated by treatment of cells with methyl-β-cyclodextrin (MβCD). Severe intestinal inflammation induced by CDT treatment was observed in high-cholesterol diet-fed mice, but not in normal diet-fed mice, indicating that cholesterol is essential for CDT intoxication.
Conclusion:
Our findings demonstrate a molecular link between Campylobacter jejuni CDT and cholesterol, which is crucial to facilitate CDT-induced pathogenesis in hosts.
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