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Updated: Apr 15, 2026

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Published on: September 11, 2020

Genotyping Streptococcus pneumoniae.

Rachael E Rayner¹, John Savill, Louise M Hafner

¹Institute of Health & Biomedical Innovation (IHBI), 60 Musk Ave, Kelvin Grove, 4059, Queensland, Australia.

Future Microbiology

|April 14, 2015

Summary

This summary is machine-generated.

Streptococcus pneumoniae genotyping is crucial for understanding this deadly pathogen. This review explores various methods, including multilocus sequence typing and multilocus variable number of tandem repeat analysis, to improve bacterial characterization.

Keywords:

MLST MLVA Streptococcus pneumoniae epidemiology genotyping

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Last Updated: Apr 15, 2026

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

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Published on: February 24, 2014

Area of Science:

Microbiology
Genetics
Epidemiology

Background:

Streptococcus pneumoniae causes significant global morbidity, mortality, and economic burden.
Current standard genotyping methods like multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) are widely used.
The development of multilocus variable number of tandem repeat analysis (MLVA) offers a rapid, less expensive, and highly discriminatory alternative.

Purpose of the Study:

To review and compare various genotyping protocols for Streptococcus pneumoniae.
To highlight limitations of existing methods, such as the lack of a universal MLVA protocol.
To discuss emerging technologies and research directions in S. pneumoniae characterization.

Main Methods:

Review of existing literature on S. pneumoniae genotyping techniques.
Comparative analysis of multilocus sequence typing (MLST), pulsed field gel electrophoresis (PFGE), and multilocus variable number of tandem repeat analysis (MLVA).
Discussion of other developed genotyping and characterization methods.

Main Results:

MLVA is a promising genotyping technique due to its discriminatory power, speed, and cost-effectiveness.
Existing MLVA protocols lack universality and may fail to amplify certain loci across all pneumococcal serotypes, leading to incomplete profiles.
Various other genotyping methods have been developed and contribute to understanding S. pneumoniae.

Conclusions:

Standardization of MLVA protocols is needed for universal application in S. pneumoniae genotyping.
Continued research and technological advancements are essential for comprehensive characterization of this pathogen.
Effective genotyping is critical for tracking and controlling the spread of Streptococcus pneumoniae infections.