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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Inhibitors of Bacterial DNA Synthesis01:28

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Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These...
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Targeted Cancer Therapies02:57

Targeted Cancer Therapies

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The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
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Targets for Drug Action: Overview01:26

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Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
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DNA Base Pairing02:27

DNA Base Pairing

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Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
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DNA Helicases00:55

DNA Helicases

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DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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Related Experiment Video

Updated: Apr 15, 2026

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin
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Therapeutic Agents Based on DNA Sequence Specific Binding.

Luke Pett1, John A Hartley, Konstantinos Kiakos

  • 1Cancer Research UK Drug- DNA Interactions Research Group, UCL Cancer Institute, Paul O'Gorman Building, 72 Huntley Street, London, WC1E 6BT, UK. luke.pett.12@ucl.ac.uk.

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New small molecules targeting DNA offer a promising strategy for cancer treatment. These agents aim for increased therapeutic activity and reduced toxicity by selectively interacting with critical genomic sequences.

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Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
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Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
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Area of Science:

  • Oncology
  • Molecular Biology
  • Medicinal Chemistry

Background:

  • DNA-interactive agents have been a cornerstone of cancer chemotherapy.
  • While molecular targeted therapies are advancing, DNA remains a crucial target for anti-cancer drugs.
  • Next-generation agents require enhanced therapeutic efficacy and minimized off-target toxicity.

Purpose of the Study:

  • To evaluate non-covalent DNA-binding small molecules as "gene-control" agents.
  • To explore the potential of sequence-selective agents for targeting critical genomic sites.
  • To review natural products and synthetic derivatives with sequence-specific DNA-covalent modification activity.

Main Methods:

  • Evaluation of non-covalent DNA binding small molecules.
  • Exploitation of inherent or engineered DNA sequence selectivity.
  • Review of natural products and synthetic derivatives acting via DNA-covalent modification.

Main Results:

  • Non-covalent DNA binding small molecules show potential as gene-control agents.
  • Sequence selectivity is key for targeting critical genomic sequences.
  • Various natural and synthetic compounds exhibit sequence-specific DNA-covalent modification.

Conclusions:

  • Non-covalent DNA-binding small molecules represent a viable strategy for next-generation cancer therapy.
  • Targeting DNA with sequence-selective agents can improve therapeutic outcomes.
  • Further research into these agents may lead to more effective and safer cancer treatments.