Related Experiment Video
Updated: Apr 15, 2026
Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
Published on: September 23, 2022
The prevalence of STL polyomavirus in stool samples from Chinese children
Insights
STL polyomavirus (STLPyV) was not found to be more prevalent in Chinese children with diarrhea compared to healthy children. This study provides the first prevalence data for STLPyV in China and its whole genome sequences.
Area of Science:
- Virology
- Gastroenterology
- Public Health
Background:
- Human polyomaviruses (HPyVs) are increasingly discovered, with many found in the gastrointestinal tract.
- The role of HPyVs, including STL polyomavirus (STLPyV), in gastroenteritis requires further investigation.
Purpose of the Study:
- To determine the prevalence of STLPyV in hospitalized children with diarrhea in China.
- To explore the potential clinical significance of STLPyV in pediatric gastroenteritis.
- To obtain whole genome sequences of STLPyV strains in China.
Main Methods:
- Screening of stool samples from 508 children with diarrhea and 271 healthy children for STLPyV.
- Detection of other common enteric viruses and HPyVs (HPyV12, NJPyV-2013) for comparison.
- Whole genome sequencing of detected STLPyV strains.
Main Results:
- STLPyV was detected in 2.2% of children with diarrhea and 3.0% of healthy children, with no significant difference in prevalence.
- STLPyV detection rates were similar between children with and without other common enteric viral infections.
- Two complete STLPyV genomes were successfully sequenced.
Conclusions:
- This is the first report on STLPyV prevalence and whole genome sequences in Chinese children.
- STLPyV does not appear to be a significant cause of gastroenteritis in the studied pediatric population in China.
- Phylogenetic analysis suggests STLPyV is geographically widespread.
Background:
Over the past 7 years, eleven novel human polyomaviruses (HPyVs) have been identified. The frequent discovery of human polyomaviruses (HPyVs) in the gastrointestinal tract and stool samples suggests a potential involvement in gastroenteritis.
Objective:
In this study we want to explore the prevalence of STL polyomavirus (STLPyV) in China and delineate the clinical role played by STLPyV.
Study Design:
Stool samples from 508 hospitalized children with diarrhea and 271 healthy children were screened to detect STLPyV. Human polyomavirus 12(HPyV12), New Jersey polyomavirus (NJPyV-2013) and six common enteric viruses (including rotaviruses, adenovirus, norovirus GI and GII, astrovirus and sapovirus) were also screened in this study.
Results:
348 of the 508 (68.5%) specimens from the hospitalized children with diarrhea contained at least 1 common enteric virus. STLPyV was identified in 11 specimens in the case group (2.2%), among which 4 specimens were negative for those common enteric viruses. STLPyV was not more prevalent among the case group than the control group (2.2% versus 3.0%; p = 0.50, χ(2) test). In case group, when common enteric viruses' positive and negative groups were compared, the difference in detection rate of STLPyV was not statistically significant (2.5% versus 2.0%; p = 0.98, χ(2) test). Two whole genome sequences of STLPyV were obtained.
Conclusions:
We are the first to report the prevalence of STLPyV in Chinese children and obtained whole genome sequences of STLPyV strains isolated in China. Our results of phylogenetic analysis support the hypothesis that STLPyV is geographically widespread.
More Related Videos
15:16Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
06:22Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
Published on: February 10, 2023
Related Concept Videos
01:27Cytomegalovirus Disease
01:18Bacterial Gastroenteritis