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Related Concept Videos

Influenza01:27

Influenza

52
Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...
52

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Influenza Virus Propagation in Embryonated Chicken Eggs
06:56

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Published on: March 19, 2015

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Influenza virus propagation in embryonated chicken eggs.

Rena Brauer1, Peter Chen2

  • 1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Women's Guild Lung Institute, Cedars-Sinai Medical Center; rena.brauer@cshs.org.

Journal of Visualized Experiments : Jove
|April 14, 2015
PubMed
Summary

This study presents a reliable and cost-effective method for propagating influenza A virus in chicken eggs, yielding high-titer viral stocks essential for research and development of new vaccines and drugs.

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Area of Science:

  • Virology
  • Microbiology
  • Immunology

Background:

  • Influenza virus causes significant annual mortality and hospitalizations in the US.
  • Emerging influenza strains necessitate continuous development of novel drugs and vaccines.
  • Reliable and cost-effective influenza virus propagation methods are crucial for laboratory research.

Purpose of the Study:

  • To provide a comprehensive protocol for influenza A virus propagation in fertile chicken eggs.
  • To establish a method for consistently yielding high-titer viral stocks.
  • To facilitate in vitro studies requiring large quantities of influenza virus.

Main Methods:

  • Serum pathogen-free (SPF) fertilized chicken eggs incubated for 10-11 days at 37°C.
  • Virus inoculation into the allantoic cavity via needle injection.
  • Harvesting allantoic fluid after 2 days incubation, followed by chilling, centrifugation, and storage at -80°C.

Main Results:

  • The protocol consistently yields high-titer influenza A virus stocks.
  • Large volumes (5-10 ml per egg) of virus-containing fluid are obtained.
  • The method is suitable for in vitro studies requiring substantial amounts of virus.

Conclusions:

  • Chicken egg propagation is a favorable method for preparing influenza virus stocks.
  • This protocol supports the ongoing need for influenza virus research and countermeasure development.
  • The method's efficiency and yield make it ideal for large-scale virus stock generation.