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Updated: Apr 15, 2026

Tracking Mouse Bone Marrow Monocytes In Vivo
12:08

Tracking Mouse Bone Marrow Monocytes In Vivo

Published on: February 27, 2015

10.1K

Tracking mouse bone marrow monocytes in vivo.

Pauline Hamon1, Mathieu Paul Rodero1, Christophe Combadière1

  • 1Centre d'Immunologie et des Maladies Infectieuses (CIMI), INSERM, U1135, CNRS, ERL 8255, Sorbonne Universités, UPMC Univ Paris 06, CR7.

Journal of Visualized Experiments : Jove
|April 14, 2015
PubMed
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Real-time imaging reveals how immune cells called monocytes travel from bone marrow into blood vessels. This study tracks monocyte migration, offering insights into immune cell movement during inflammation.

Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • Immune cell trafficking is crucial for physiological and pathological processes.
  • Monocytes, key immune cells, originate in bone marrow and circulate in blood with short half-lives.
  • Understanding monocyte migration from bone marrow is vital for inflammatory disease research.

Purpose of the Study:

  • To visualize and quantify in vivo monocyte migration within the bone marrow.
  • To investigate the biomechanical events governing monocyte egress into the vasculature.
  • To explore cell-to-cell interactions during monocyte trafficking.

Main Methods:

  • Utilized in vivo time-lapse imaging with two-photon microscopy.
  • Employed the Csf1r-Gal4VP16/UAS-ECFP (MacBlue) mouse model for myeloid-specific fluorescent labeling.

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Last Updated: Apr 15, 2026

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  • Combined with vascular network labeling for comprehensive tracking.

    • Main Results:

    • Enabled real-time tracking of individual medullary monocytes.
    • Quantified monocyte migratory behavior within bone marrow parenchyma and vasculature.
    • Observed and analyzed cell-to-cell interactions during trafficking.

    Conclusions:

    • The study provides novel insights into bone marrow monocyte subset biology.
    • This imaging approach allows detailed analysis of monocyte migration dynamics.
    • Offers a platform to study monocyte behavior in pathological conditions.