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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...

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Related Experiment Video

Updated: Apr 15, 2026

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

Rapid reverse genetic screening using CRISPR in zebrafish.

Arish N Shah¹, Crystal F Davey¹, Alex C Whitebirch¹

¹Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

|April 14, 2015

Summary

This summary is machine-generated.

CRISPR technology efficiently mutated zebrafish genes to identify new genes involved in biological processes. This powerful reverse genetic screening method advances vertebrate systems research.

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Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

Related Experiment Videos

Last Updated: Apr 15, 2026

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

Area of Science:

Genetics
Molecular Biology
Developmental Biology

Background:

Understanding gene function is essential for deciphering biological processes.
Zebrafish (Danio rerio) are a key vertebrate model for genetic studies.

Purpose of the Study:

To develop and validate CRISPR (clustered regularly interspaced short palindromic repeats) as an efficient reverse genetic screening tool in zebrafish.
To identify novel genes involved in vertebrate biological processes using CRISPR screening.

Main Methods:

Engineered CRISPR system with optimized Cas9 mRNA and multiplexed single guide RNAs (sgRNAs) for efficient gene mutation.
Proof-of-concept pooled screen of 48 loci in zebrafish embryos.
Deep sequencing to assess screening efficiency and identify mutations.

Main Results:

Optimized CRISPR system successfully phenocopied known zebrafish mutants.
Identified two novel genes crucial for electrical-synapse formation.
Achieved 90% screening efficiency across targeted loci.

Conclusions:

CRISPR is a powerful and efficient in vivo reverse genetic screening strategy for vertebrate systems.
This approach accelerates the discovery of genes involved in fundamental biological processes.