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Endogenous adenosine can reduce epileptiform activity in the human epileptogenic cortex maintained in vitro
G Kostopoulos1, C Drapeau, M Avoli
1Montreal Neurological Institute, McGill University, Que., Canada.
Neuroscience Letters
|November 20, 1989
Summary
Adenosine and its analog, 2-Cl-adenosine, significantly reduce epileptiform activity in human brain tissue. This suggests a purinergic mechanism can control seizures in the human epileptogenic neocortex.
Area of Science:
- Neuroscience
- Pharmacology
- Epilepsy Research
Background:
- Epileptogenesis involves abnormal neuronal excitability.
- The role of adenosine in human epileptogenic neocortex requires further elucidation.
Purpose of the Study:
- To investigate the effects of adenosine and its analogs on Mg2+-free epileptogenesis in human neocortex.
- To explore the potential of purinergic mechanisms in controlling epileptiform activity.
Main Methods:
- In vitro study using slices of human epileptogenic neocortex.
- Extracellular recordings to monitor stimulus-induced and spontaneous epileptiform activity.
- Application of adenosine, 2-Cl-adenosine, and nitrobenzylthioinosine.
Main Results:
- Adenosine (40-50 microM) decreased spontaneous epileptiform discharges by 30-90%.
- 2-Cl-adenosine (0.3-3 microM) reduced discharge frequency by over 75% and blocked activity.
- Adenosine uptake inhibition indicated endogenous adenosine influences epileptogenicity.
Conclusions:
- Purinergic mechanisms, mediated by adenosine, can control Mg2+-free epileptiform activity in the human epileptogenic neocortex.
- Adenosine and its analogs show potential as therapeutic agents for epilepsy.