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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Apr 15, 2026

Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall

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A method for immunolabeling neurons in intact cuticularized insect appendages.

Erica Ehrhardt¹, Tatjana Kleele, George Boyan

¹Graduate School of Systemic Neuroscience, Biocenter, Ludwig-Maximilians-Universität, Grosshadernerstrasse 2, 82152, Planegg-Martinsried, Germany.

Development Genes and Evolution

|April 15, 2015

Summary

Researchers developed a sonication method to improve immunolabeling in grasshopper antennae, enabling detailed study of the peripheral nervous system during development.

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Last Updated: Apr 15, 2026

Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall

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Published on: June 13, 2012

Area of Science:

Neuroscience
Developmental Biology
Entomology

Background:

Grasshopper antennae develop complex nerve pathways via pioneer neurons and sensory cell clusters.
Immunolabeling is crucial for studying nervous system development but is hindered by cuticle formation in later stages.

Purpose of the Study:

To develop a reliable method for immunolabeling the nervous system of cuticularized grasshopper antennae.
To enable reconstruction of the peripheral nervous system throughout development using confocal microscopy.

Main Methods:

Modified a sonication procedure to create porous cuticles in late embryonic and first instar grasshopper antennae.
Applied immunolabeling to sonicated antennae and compared results with sectioned controls.
Examined expression patterns of neuron-specific, microtubule-specific, and proliferative cell-specific labels.

Main Results:

Sonication effectively rendered cuticularized antennae porous to antibodies without compromising cellular organization.
Axon projections and sensory cluster organization remained intact in sonicated antennae.
Molecular epitope expression patterns were consistent with earlier developmental stages, indicating conserved molecular markers.

Conclusions:

Sonication is a viable method for reliable immunolabeling in intact, cuticularized insect appendages.
This technique facilitates direct reconstruction of the peripheral nervous system via confocal microscopy throughout development.
The study validates conserved molecular epitopes crucial for understanding neurodevelopment in grasshoppers.