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Purification of a High Molecular Mass Protein in Streptococcus mutans
Published on: September 14, 2019
Purification and characterization of a novel glycoprotein from Streptomyces sp. ZX01
Guoqiang Zhang1, Lirong Han1, Guifeng Zhang2
1Research & Development Center of Biorational Pesticide, Northwest A & F University, Yangling 712100, Shaanxi, China.
Abstract:
A novel glycoprotein GP-1 with antiviral activity against plant virus was isolated from the fermentation broth of the actinomycete Streptomyces sp. ZX01. MALDI-TOF-MS proved that molecular weight of GP-1 approximately was 8.5 kDa. GP-1 was a heat-sensitive glycoprotein with decreasing antiviral activity after treated from 80 °C to 100 °C for 30 min. GP-1 contained 40.23% carbohydrate with N-linked and O-linked glycan. FT-IR and NMR spectra proved that GP-1 contained protein and carbohydrate portions with α-D-(1,6)-glucose residues. Circular dichroism revealed that GP-1 was a glycoprotein with a large unordered content. Moreover, protein sequencing was predicted by using MALDI-TOF-MS and Mascot search. These results suggested that glycoprotein GP-1 could be used as a novel natural antiviral agent in agricultural industry.
Insights
A novel glycoprotein, GP-1, isolated from Streptomyces sp. ZX01 exhibits potent antiviral activity against plant viruses. This heat-sensitive glycoprotein shows promise as a natural antiviral agent for agricultural applications.
Area of Science:
- Microbiology
- Biochemistry
- Plant Pathology
Background:
- Plant viruses cause significant agricultural losses worldwide.
- There is a continuous need for novel antiviral agents, particularly natural compounds.
- Actinomycetes are a known source of bioactive molecules.
Purpose of the Study:
- To isolate and characterize a novel glycoprotein with antiviral activity from Streptomyces sp. ZX01.
- To evaluate the potential of this glycoprotein as a natural antiviral agent for agricultural use.
Main Methods:
- Isolation of glycoprotein GP-1 from Streptomyces sp. ZX01 fermentation broth.
- Characterization using MALDI-TOF-MS, FT-IR, NMR, and Circular Dichroism.
- Assessment of heat sensitivity and antiviral activity.
Main Results:
- A novel glycoprotein, GP-1 (approx. 8.5 kDa), was isolated.
- GP-1 demonstrated heat sensitivity, with reduced activity above 80°C.
- The glycoprotein contains protein and carbohydrate portions, including α-D-(1,6)-glucose residues and N-linked/O-linked glycans.
Conclusions:
- Glycoprotein GP-1 possesses significant antiviral activity against plant viruses.
- Its natural origin and characterized properties suggest potential as a novel agricultural antiviral agent.
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