Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent treatment outcomes of cervical intraepithelial neoplasia in south western Uganda: a six year retrospective study.

Gynecologic oncology reports·2026
Same author

Detection of mutant cancer-derived circRNAs in extracellular vesicles by rolling circle amplification.

Molecular therapy. Nucleic acids·2026
Same author

Hydrogel-based multiplexed high-risk human papillomavirus DNA/RNA detection for cervical cancer screening.

Biosensors & bioelectronics: X·2026
Same author

Molecular Analysis of Single Tumor-Derived Extracellular Vesicles with Improved Robustness and Accuracy.

Analytical chemistry·2026
Same author

Self-amplifying CRISPR-based one-pot ultrasensitive testing for rapid SARS-CoV-2 and its variant detection.

Biosensors & bioelectronics·2026
Same author

Smartphone-based preeclampsia diagnosis through detection of dual biomarkers - nephrin and podocalyxin.

Biosensors & bioelectronics·2026

Related Experiment Video

Updated: Apr 15, 2026

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection
06:00

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

2.3K

Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone.

Hyungsoon Im1, Cesar M Castro2, Huilin Shao3

  • 1Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114; Department of Radiology, Massachusetts General Hospital, Boston, MA 02114;

Proceedings of the National Academy of Sciences of the United States of America
|April 15, 2015
PubMed
Summary
This summary is machine-generated.

Smartphone-based digital diffraction diagnosis (D3) enables rapid molecular and cellular diagnostics using microbeads and smartphone imaging. This innovative approach shows promise for point-of-care testing in resource-limited settings.

Keywords:
cancer diagnosticscervical cancermolecular sensingpoint-of-care diagnosticssmartphone

More Related Videos

Real-time Tracking of DNA Fragment Separation by Smartphone
06:58

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

15.4K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.5K

Related Experiment Videos

Last Updated: Apr 15, 2026

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection
06:00

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

2.3K
Real-time Tracking of DNA Fragment Separation by Smartphone
06:58

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

15.4K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.5K

Area of Science:

  • Biomedical engineering
  • Molecular diagnostics
  • Point-of-care testing

Background:

  • Smartphones offer accessible platforms for point-of-care (POC) diagnostics due to their ubiquity and integrated sensors.
  • Implementing molecular diagnostics on smartphones has been a significant challenge, particularly in resource-limited environments.

Purpose of the Study:

  • To develop a novel diffraction-based method for molecular and cellular diagnostics compatible with smartphones.
  • To evaluate the efficacy of this system for detecting precancerous/cancerous cervical cells and human papillomavirus (HPV) DNA.

Main Methods:

  • The digital diffraction diagnosis (D3) system utilizes microbeads to create unique diffraction patterns.
  • These patterns are captured by smartphone cameras and analyzed via a remote server.
  • The D3 platform was applied to cervical specimens for cell screening and HPV DNA detection.

Main Results:

  • The D3 assay provided diagnostic readouts in under 45 minutes.
  • Results demonstrated excellent agreement with established gold-standard pathology and HPV testing methods.
  • The system successfully screened for precancerous/cancerous cervical cells and detected HPV DNA.

Conclusions:

  • The D3 system offers a feasible approach for smartphone-based molecular and cellular diagnostics.
  • This technology has significant potential for global health applications, addressing limitations in medical access and diagnostic turnaround times.
  • The diffraction-based method overcomes previous challenges in implementing molecular diagnostics on mobile platforms.