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Listeria monocytogenes - characterization of strains isolated from clinical severe cases

A M Borcan1, S Huhulescu2, A Munteanu3

  • 1"Matei Bals" National Institute for Infectious Diseases, Bucharest, Romania.

Journal of Medicine and Life
|April 15, 2015
PubMed

Insights

Most Listeria monocytogenes clinical isolates in Romania showed resistance to ciprofloxacin and other antibiotics. Two common molecular types were identified, potentially linked to increased severity in immunocompromised patients.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Clinical Research

Background:

  • Listeria monocytogenes is an emerging pathogen causing severe foodborne illnesses and outbreaks.
  • Clinical isolates require ongoing surveillance for antimicrobial susceptibility patterns.

Purpose of the Study:

  • To investigate the in vitro antimicrobial susceptibility of clinical Listeria monocytogenes strains.
  • To identify molecular types of Listeria monocytogenes prevalent in clinical settings.

Main Methods:

  • Disk diffusion method was used to test 26 clinical isolates against 15 antimicrobial agents.
  • Multiplex PCR was employed for serological and molecular typing.

Main Results:

  • No multidrug-resistant strains were detected, but 72% of isolates were resistant to at least one antibiotic.
  • A significant finding was the resistance of 18 isolates to ciprofloxacin, particularly noted in strains from Romania.
  • Two prevalent molecular types, 1/2 a, 3a and 1/2 b, 3b, were identified.

Conclusions:

  • Clinical Listeria monocytogenes isolates in Romania exhibit concerning resistance patterns, especially to ciprofloxacin.
  • Identified molecular types may correlate with increased pathogenicity in immunocompromised individuals.
Keywords:
Listeria monocytogenesserotypingsevere clinical infectionstrainssubtyping

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