Related Experiment Video
Updated: Apr 15, 2026
Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia
Published on: April 7, 2023
HPLC-MS/MS method for the determination of four lignans from Phyllanthus urinaria L. in rat plasma and its
Hongyan Fan1, Wei Zhang, Jing Wang
1MOE Key Laboratory of Drug Quality Control & Pharmacoigilance, China Pharmaceutical University, Nanjing 210009, China.
Background:
Phyllanthus urinaria L. showed significant hepatoprotective and anti-HBV activities. A sensitive and rapid HPLC-MS/MS method has been developed and validated for the simultaneous determination of hypophyllanthin, phyllanthin, nirtetralin and niranthin from Phyllanthus urinaria L. in rat plasma. The analytes were extracted from rat plasma with n-hexane/isopropanol and diazepam was used as the internal standard.
Results:
The calibration curves were linear (r > 0.9971) over a concentration range of 2-1000 ng/ml for hypophyllanthin and nirtetralin, and 1-1000 ng/ml for phyllanthin and niranthin. The precision, accuracy, recovery and stability were deemed acceptable.
Conclusion:
The HPLC-MS/MS method was successfully applied to a PK study of the four lignans in rats after oral administration of Phyllanthus urinaria L. extract.
More Related Videos
09:01Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
Published on: June 26, 2020
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022